**Non-destructive marking with plugin ObjectJ**

http://simon.bio.uva.nl/objectj
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  * ObjectJ supports graphical vector objects that non-destructively mark images on a transparent layer.

  * Vector objects can display points or paths with sub-pixel resolution; they can be placed manually or by macro commands.

  * Composite objects can encapsulate different color-coded marker structures in order to bundle features that belong together.

  * ObjectJ provides back-and-forth navigation between results and images. The results table supports statistics, sorting, color coding, qualifying and macro access.

  * A single file such as "MyProject.ojj" is used to maintain the link between markers and measurements across many images or stacks.