====== Stitch data from MetaMorph 7 "Screen Acquisition" module ====== Reassembles tiling data acquired by MetaMorph into stacks and hyperstacks. MetaMorph 7's Screen Acquisition (screenacq) does not natively support time and Z acquisitions from it's UI or journal functions. Therefore it's easier to assemble the 5D experiment (x,y,z,t,c) with FIJI including stitch TIF files together with overlap. === Output === {{:macro:review_screen_data_output.png|Hyperstack output from running the "Stitch Raw Data" macro tool}} === Requirements === [[http://fiji.sc/wiki/index.php/Downloads|FIJI]], since this macro makes use of: * ImageJ 1.46c or later * [[http://fiji.sc/wiki/index.php/Image_Stitching|Grid/Collection stitching plugin]] === Installation === Download "Review Screen Data.txt" to the >Macros>Toolsets folder. It will show up in the ">>" toolsets dropdown under the name of the text file, "Review Screen Data". ^ Filename ^ Last Modified ^ |''{{:macro:rss:07a:review_screen_data.txt|Review Screen Data.txt}}''| 2013/02/04 | === License === Public Domain === Known Bugs === - Window flicker for each well site while constructing hyperstack. * ImageJ has a bug where it throws a ''NullPointerException'' when using ''BatchMode'' to ''run("Stack to Hyperstack...", ...)'' followed by ''close()''. * As a workaround these functions need to be run outside of ''BatchMode'', which causes the ficker of the window appearing and closing. * Wayne Rasband is aware of this bug. - Crash during macro stitching leaves ''*.HTD'' metadata files renamed as ''*.HTD.bak''. * Re-run the macro to fix. * This renaming for is necessary to prevent the Bioformats reader from confusing MetaMorph ''*.HTD'' files to be Applied Precision files. === Change History === * 2013.02.04 v0.7a * Fix "Stack required" error by skipping hyperstack assembly when not needed * 2013.02.03 v0.6 * Fix for dot characters in dataset file naming * 2012.10.21 v0.5 * Add Z projection macro * Add toolbar icons for multiple macros * Cleanup loop variables * 2012.10.18 v0.4 * Fix single channel dataset file naming * 2012.10.15 v0.3 * Initial public release * Add user input validation of Z slices * Workaround ImageJ BatchMode bug with creating hyperstack * Fix progress bar index * 2012.10.14 v0.2 * Add progress bar * Add License * Create hyperstacks * 2012.10.12 v0.1 * Initial development release === Future Additions / Improvements === * LOCI tools Bioformats should add support for Molecular Devices ''*.HTD'' data. * Multi-well viewer macro to directly open a hyperstack by clicking the well position.