====== MetroloJ ======
**This plugin helps keeping track of microscopes' performances by extracting 4 relevant estimators out of standardized images, acquired from test samples.**
===== Author =====
Fabrice P. Cordelières, Bordeaux Imaging Center (France). Fabrice.Cordelieres at gmail dot com
Cédric Matthews, CNRS - IBDML - UMR 6216,Marseille (France). cedric.matthews at ibdml.univmed.fr
This plugin has been created as part of the metrology workgroup work, within the framework of the [[http://rtmfm.cnrs.fr/|"French technological network for multidimensional fluorescence microscopies" (RT- MFM)]], supported by the [[http://www.mrct.cnrs.fr/|Mission ressources et compétences technologiques du CNRS" (MRCT)]]".
===== Features =====
This plugin allows measuring relevant parameters which helps testing, following and comparing microscopes performances. This is achieved by extracting four indicators out of standardized images, acquired from standardized samples: the estimation of the detector sensitivity , the evaluation of the field illumination homogeneity, the system resolution, and finally the characterization of its spectral registration.
===== Description =====
This plugin is composed of five parts:
* Generate CV report
* Generate field illumination report
* Generate PSF report
* Generate axial resolution report
* Generate co-alignement report
Please refer to the {{:plugin:analysis:metroloj:metroloj.pdf|plugin's manual}} for more details.
===== References/Citation =====
When using the “MetroloJ” plugin for publication, please refer to {{:plugin:analysis:metroloj:matthews_cordelieres_-_imagej_user_developer_conference_-_2010.pdf|Cédric Matthews and Fabrice P. Cordelieres, MetroloJ : an ImageJ plugin to help monitor microscopes' health, in ImageJ User & Developer Conference 2010 proceedings}}, to this webpage and of course to ImageJ, as explained in the [[http://rsbweb.nih.gov/ij/docs/faqs.html|FAQ section, on ImageJ’s website]]. A copy of your paper being sent to both our e-mail address would also be greatly appreciated !
===== Installation =====
Simply download {{:plugin:analysis:metroloj:metroloj_.jar|}} to the Plugins folder of ImageJ, use the "Help/Update Menus" function to refresh the plugins' menu.
**Reports are generated as pdf files, using currently the {{:plugin:analysis:metroloj:itext-2.1.4.jar|iText library v2.1.4}} ([[http://itextpdf.com/download.php|Newer versions are available from iText website, but are not yet compatible with MetroloJ]]) which should be downloaded to the Plugins folder of ImageJ.**
If you get an error message stating "java.lang.NoClassDefFoundError: com/lowagie/textDocumentationException...", it means you are missing the {{:plugin:analysis:metroloj:itext-2.1.4.jar|iText library v2.1.4}}. Download it and save it to your plugins' folder.
===== Downloads =====
{{:plugin:analysis:metroloj:metroloj_.jar|The plugin is available from here}}
{{:plugin:analysis:metroloj:metroloj.pdf|The plugin's manual is available from here}}
===== License =====
This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 3 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program. If not, see [[http://www.gnu.org/licenses/|http://www.gnu.org/licenses/]].
Copyright (C) 2011 Fabrice P. Cordelières/Cédric Matthews
===== Changelog =====
22/04/11: First public release
06/07/17: Correction of a bug in the field illumination report where wrong intensity was reported for the bottom-right corner