Neurite-J is an ImageJ (http://rsbweb.nih.gov/ij/index.html) plug-in that works as an adaption of the Sholl method providing a semi-automatic tool for neurite outgrowth quantification in organotypic cultures. Neurite-J gives a good description of neurite growth providing counts of neurite number and neurite area at different distances from the organotypic explant.
Neurite-J is developed in the Neuroplasticity and Repair Group of the University Autonomous of Barcenlona (UAB) by Dr. Abel Torres Espín.
*.java:
Download the java file into …/ImageJ/pluguins/ folder. We recommends create a NeuriteJ folder and save the file inside. To install Neurite-J use the Plugins>Install… command in ImageJ interface and select the java file.
*.jar:
Download the jar file into your computer. To install Neurite-J drag and drop the jar file into ImageJ window. Save the jar file into …/ImageJ/pluguins/ folder. A new menu call Neurite-J will appears in the Plugins menu of ImageJ.
Last version v1.1 (compiled) (neurite_j11.jar). A compatibility issues with java 8 and ImageJ compiler have been solved. This version of the plug-in is already compiled in a *.jar file. Version compatible with ImageJ 1.49 bundled with java 1.6 or java 1.8. To access the code download the source code (*.java)
Last version v1.1 (source code) (neurite_j11.java).This version of the plug-in works under ImageJ 1.49 bundled with java 1.6. Later versions have not been tested.
Neurite-J v1.01 (neurite_j101.java). This version of the plug-in works under ImageJ 1.47, 1.48 and 1.49 versions for Mac Os X, Linux and Windows systems (later versions have not been tested).
Neurite-J v1.0 (neurite_j148.java) is the first available version of the Neurite-J plug-in for ImageJ software. This version of the plug-in works under ImageJ 1.47 and 1.48 versions for Mac Os X, Linux and Windows systems (later versions have not been tested).
Plug-in validation published in the Journal of Neuroscience Methods: http://authors.elsevier.com/a/1PalebXTOZ4ZB
Torres-Espín A, Santos D, González-Pérez F, del Valle J, Navarro X. Neurite-J: An Image-J plug-in for axonal analisys in organotypic cultures. J Neurosci Meth 236:26–39.
For any suggestions, problems or errors communication, don't forget to send an e-mail to atpspin@gmail.com with NeuriteJ as a subject.