//This macro was designed to measure the number of granules and area in GFP-LC3 transfected cells //magnification. You may choose to select individual cells to measure area and count granules, but the program will not correct for scale // Do not select the cell with the tool function if measuring areas, express result as average area of granules for population // Creator: Ruben Dagda, Chu-Lab 030207 macro "Measure Granules [F5]" { print("Granule GFP-LC3 macro, Ruben Dagda 03/02/07"); open(); run("RGB Split"); run("Set Measurements...", "area mean integrated limit display redirect=None decimal=3"); setTool(2); macro "Process RGB Images [F6]" { run("Copy"); run("Duplicate...","\n"); run("Paste"); run("Invert"); run("Sharpen"); setAutoThreshold(); //run("Threshold..."); } macro "Measure [F7]" { run("Clear Results"); run("Set Measurements...", "area circularity redirect=None decimal=3"); run("Analyze Particles...", "display results summarize size distribution minimum=11 maximum=4000 bins=20 show=Ellipses display summarize"); for (i=0; i0) { selectImage(nImages); close(); } }