run("MRI Stack (528K)");
run("32-bit");
run("3D Fast Filters","filter=Sobel radius_x_pix=2.0 radius_y_pix=2.0 radius_z_pix=2.0 Nb_cpus=12");
run("Invert", "stack");
run("3D Watershed", "seeds_threshold=0 image_threshold=0 image=3D_Sobel seeds=Automatic radius=4");
setSlice(nSlices/2);
run("Enhance Contrast", "saturated=0.35");
run("3-3-2 RGB");