// use plugins/macros/record to record the necessary opening and channel splitting // 3 images are opened in this example : labels.tif with segmented object, C1-signal.tif and C2-signaltif with signal to quantify // select measurments in Manager3D Options, select Plugins/Record to record run("3D Manager Options", "volume integrated_density mean_grey_value std_dev_grey_value mode_grey_value minimum_grey_value maximum_grey_value distance_between_centers=10 distance_max_contact=1.80 drawing=Contour");// run the manager 3D and add image run("3D Manager"); // select the iamge with the labelled objects selectWindow("labels.tif"); Ext.Manager3D_AddImage(); // do some quantifications, save measurements and close window selectWindow("C1-signal.tif"); Ext.Manager3D_Quantif(); Ext.Manager3D_SaveResult("Q","/home/boudier.t/C1-Quantif3D.csv"); Ext.Manager3D_CloseResult("Q"); selectWindow("C2-signal.tif"); Ext.Manager3D_Quantif(); Ext.Manager3D_SaveResult("Q","/home/boudier.t/C2-Quantif3D.csv"); Ext.Manager3D_CloseResult("Q"); // Use Q for the Quantification window, D for distances, C for colocalisation, L or V for list voxels and A for A windows // if list is not visible please refresh list by using Deselect //Ext.Manager3D_Select(0); //Ext.Manager3D_DeselectAll();