This software was designed to ease the determination of unitary optical density vectors used in the color decovolution plugin written by Gabriel Landini https://blog.bham.ac.uk/intellimic/g-landini-software/colour-deconvolution-2/ and based on the original publication by Ruifrok and Johnston:Quantification of histochemical staining by color deconvolution. The major idea of this imageJ plugin relies on the fact that 3D optical density unitary vectors (which are the core of the colour deconvolution algorithm) have constant radius = 1. Therefore, one can transform 3D vectors to 2D vectors by using polar coordinates and getting rid of the radius. 2D optical density vectors can then be mapped on a stereographic projection map. Please refers to: Color deconvolution. Optimizing handling of 3D unitary optical density vectors with polar coordinates.
Gilbert Bigras, Cross Cancer Institute, University of Alberta, Edmonton Canada (firstname.lastname@example.org)
The first window corresponds to the main menu. Two major actions are possible:
The plugin works well with big screen. You can move and resize each window and especially the stereographic map by clicking '+' and '-' signs. By doing this the vector V1 will be accidentally modified. Just click on the button 'reset all'.
If you click 'main menu' button and click 'LAUNCH vectors selector' button again all windows will be appropriately scaled
Simply download unitvectorsselection_.jar to the Plugins folder of ImageJ, restart ImageJ and use the “UnitVectorsSelection” command in the Plugins menu. For your convenience you can download the following five pictures to try this plugin. Three are three pure stains (DAB, Hematoxylin and Red) and two composite pictures to be deconvoluted. Click on small individual image and save the larger version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with the programs; if not, write to the Free Software Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA
Free software: you can redistribute it and or modify it under the terms of the GNU General Public license as published by the Free Software Foundation; either version 2 of the license, or (at your option) any later version.
First release: December 10, 2011
Additional precisions: March 24, 2016