A macro to determine image focal quality image-wide (not ROI-wide)

// A macro to determine image focal quality image-wide (not ROI-wide)
// Based on algorithm F-2 "Squared Gradient"
// In: Sun et al., 2004. MICROSCOPY RESEARCH AND TECHNIQUE 65, 139–149.
// 
// Version: 0.1
// Date: 14/07/2006
// Author: Andy Weller
 
macro "Squared_Gradient" {
W = getWidth();
H = getHeight();
sqGrad = 0; // Set to 0 which is out of focus
for (j=0; j<H; j++) {
   for (i=0; i<W; i++) {
      p = getPixel(i,j);
      p1 = getPixel(i+1,j);
      sqGrad += (p1-p)*(p1-p); // Maximum value is best-focused, decreasing as defocus increases
      }
   }
print(sqGrad); // This can also (should) be changed to return(sqGrad)
}

by Andy Weller — last modified 2006-07-14 20:54