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SarConfoCal (Simultaneous Fluorescence and Sarcomere Length Measurements from LSCM Images)
SarConfoCal is used to analyse scanlines and multi-channel (fluorescence and transmission) from Laser Scanning Confocal Microscopy (LSCM) images of myocytes, then it plots the fluorescence signal and the sarcomere length of each line versus time.
Francois Gannier (francois.gannier at univ-tours.fr)
Come Pasqualin (come.pasqualin at univ-tours.fr)
An article on this work is in press in Bioinformatics (doi: 10.1093/bioinformatics/btw754)
Download SarConfoCal.ijm and copy it into the “ImageJ\macros\toolsets” folder. \\Start ImageJ or restart it if already opened. \\In the “More tools” menu (») of the toolbar, select “SarConfoCal”. A new set of buttons should now be present on the right side of the toolbar, as shown in the top figure below. SarConfoCal is now ready to use.
This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation (http://www.gnu.org/licenses/gpl.txt)
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.