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TAPAS (Towards an Automated Processing and Analysis System) is a framework for processing and analysis workflow for multi-dimensional images. A workflow is a series of modules linked together to process an image, each module should perform one simple task.

The first idea is to design a framework where users can share the pipeline as simple text file. The second idea is to create a simple programming template so developers can create their own module.

Finally, the pipeline should be separated from the set of images to process, so a same pipeline can be used for different images. The results of the processing and the analysis should be stored along with the processed images.

Here we propose modules to process and analyze images stored in an OMERO database, but the system should work fine with files stored locally or on a server.

Thomas Boudier

Basically we will propose modules from the 3D ImageJ Suite, ImageJ/Fiji, along with other modules.

As for stable version 0.5 the following modules are available :

• Input/Output to files (using BioFormats)
• Input/Output to OMERO
• 3D filtering
• Auto-threshold, hysteresis and Iterative thresholding
• 3D labeling
• Objects measurements, signal quantification and numbering
• Colocalisation and distances
• Calling ImageJ macros

1. Install ImageJ or Fiji.
2. Install 3D ImageJ Suite. It is also available as a update site in Fiji. ImageScience should be installed too, either from here or via the update site in Fiji.
3. Install bioformats, either by downloading bioformats_package.jar or by enabling the update site in Fiji.
4. If you have an OMERO server, download OMERO-insight, unzip OMERO.insight-ij-xxx.zip into the plugins directory of ImageJ or Fiji. The version number should match the version number of your server. Please do not install OMERO-insight from the Fiji update site.
5. Download bundle-tapas0.51.zip and unzip it into the ImageJ or Fiji directory.

TAPAS was tested successfully with OMERO.insight 5.5.5 and Bioformats 6.0.1.

1. If you want to process data stored on an Omero server, connect first to your server using TAPAS Connect inside the TAPAS menu.
2. Create your pipeline in a text editor, check available tapas modules and how to use it in the documentation provided.
3. Run TAPAS OMERO if you want to process data from Omero or TAPAS FILE for data on files.

A list of tutorials :

• Basic Input/Ouptput functionalities here.
• Create your first processing pipeline here.
• Segmentation modules here.
• Measurement modules here.
• Signal quantification and numbering modules here.
• Co-localisation here.
• How to use the analyzeParticles module for segmentation and measurement here.
• How to quantify layers distribution here.

The list of available modules is described in TapasModules0.51.pdf.

2018 Whitehead, L., Wimmer, V., Lafouresse, F., Ratnayake, D., Currie, P., Groom, J., Rogers, K. and Boudier, T. Towards an Automated Processing and Analysis System for multi-dimensional light-sheet microscopy big data using ImageJ and OMERO. International Microscopy Congress IMC 19. (pdf)

Version 0.5 and later are under GPL distribution (see license). Source code is available on github : tapas-core, tapas-plugins.

• 19/09/2019 V0.51 : minor change, new module load attachment from Omero
• 29/08/2019 V0.5 : stable version. New modules (see list)
• 06/05/2019 V0.41beta : fix bug in input/output on Windows
• 05/03/2019 V0.4beta : channels and frames now start at 1, new binning option in Omero Load, module 3dfilters is renamed filters and has parameter radxy (instead of radx and rady), new tutorials on measurements and quantification
• 01/02/2019 V0.3beta : first beta release